Table 1 Primer sets and PCR conditions for DNA amplification of Plasmodium species

Plasmodium spp.

PgMt19F3 5′-TCG CTT CTA ACG GTG AAC-3′

PgMt19B3 5′-AAT TGA TAG TAT CAG CTA TCC ATA G-3′

95 °C, 15′; 5 cycles [95 °C 10″, 62 °C 30″, 72 °C 15″]; 45 cycles [95 °C 10″, 62 °C 10″, 72 °C 15″] 72 °C, 10′

P. falciparum forward

5′-AAC AGA CGG GTA GTC ATG ATT GAG-3′

95 °C, 15′; 43 cycles [95 °C 45″, 60 °C 90″, 72 °C 45″]; 72 °C, 10′

P. malariae forward

5′-CGT TAA GAA TAA ACG CCA AGC G-3′

P. ovale forward

5′-CTG TTC TTT GCA TTC CTT ATG C-3′

P. vivax forward

5′-CGG CTT GGA AGT CCT TGT-3′

Species-specific reverse

5′-GTA TCT GAT CGT CTT CAC TCC C-3′