Fig. 7

Treatment with different inhibitors, Calpeptin and Gambogoc Acid. a ItG early trophozoite stage pRBC were treated with/without calpeptin, a calpain inhibitor for 1 h before TNF stimulation, and the expression of PfAB4 and a 90 kDa protein reacting with AB4 antibodies measured. The 100 µM calpeptin treatment caused a block of PfAB4 degradation. b ItG early trophozoite stage pRBC were treated with/without Gambogic acid (Gam), a HSP90 inhibitor overnight before TNF stimulation. The expression of PfAB4 and a 90 kDa protein reacting with AB4 antibodies was measured. Gam 0.5, 1.0 and 1.5 µM inhibited the 90 kDa protein expression and reduced the effect on PfAB4 caused by TNF