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Fig. 2 | Malaria Journal

Fig. 2

From: Alternative methods for the Plasmodium falciparum artemisinin ring-stage survival assay with increased simplicity and parasite stage-specificity

Fig. 2

Preparations for drug pulse in filtration and sorbitol-only assays. a Steps for filtration RSA are depicted. Late-stage, segmented schizonts are collected, pushed through a 1.2 µm filter, incubated, washed, incubated again, and finally incubated with either 700 nM DHA in 0.1 % DMSO or 0.1 % DMSO only. b Sorbitol-only RSA involves a single step. Early post-invasion rings (approximately 3 h old) are collected, synchronized with sorbitol to remove remaining schizonts, and plated at 1 % parasitaemia with either 700 nM DHA in 0.1 % DMSO or 0.1 % DMSO

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