Variables | Percoll gradient RSA [8] | Sorbitol-only RSA | Filtration RSA |
---|---|---|---|
Chemicals required during RSAa | Heparin, percoll, sorbitol | Sorbitol | None |
Volume of parasite culture needed | ≥300 µL packed RBCs at 2 % parasitemia | 40 µL packed RBCs at 1 % parasitemia | ≥1.5 mL packed RBCs at 2–3 % parasitemia |
Number of steps until completion | 17 | 10 | 12 |
Preparation time, before drug pulse | 4.5 h | 30 min | 3 h |
Total time of parasite incubation from drug pulse | 66 h | 66 h | 66 or 90 h, if DMSO <0.40 % at 66 h |
Stage distribution at time of drug pulse | 80 % rings, 20 % trophozoites and schizonts | Mixture of young and older rings | 3 h rings only |
Presence of pyknotic parasites in read out | Yes | Yes | No |