Identification of blood meal sources in the main African malaria mosquito vector by MALDI-TOF MS

Niare, Sirama; Berenger, Jean-Michel; Dieme, Constentin; Doumbo, Ogobara; Raoult, Didier; Parola, Philippe; Almeras, Lionel

doi:10.1186/s12936-016-1152-6

Malaria Journal

Table 1 Mosquitoes used for reference database of MALDI-TOF spectra establishment and blind tests according to post-blood feeding period and blood meal source

From: Identification of blood meal sources in the main African malaria mosquito vector by MALDI-TOF MS

Mosquito species^a	Source of blood meal	Post-blood feeding period (hours)	Number of specimens used to create the database^b	Number of specimens used for blind test	High LSVs obtained from blind tests against database^c	Vertebrate species identification of blood origin^d
An. gambiae S	Human	1	3	2	[2.540–2.624] (2)	Human
An. gambiae S	Human	12	3	2	[2.799–2.854] (2)	Human
An. gambiae S	Human	24	3	2	[2.725–2.798] (2)	Human
An. gambiae S	Human	36	0	5	[2.053–2.229] (3)	Human
					[1.153–1.711] (2)	/
An. gambiae S	Human	48	0	5	[1.155–1.327] (5)	/
An. gambiae S	Human	60	0	5	[0.900–1.489] (5)	/
Ae. albopictus	Human	1	3	2	[2.743–2.799] (2)	Human
Ae. albopictus	Human	12	3	2	[2.759–2.863] (2)	Human
Ae. albopictus	Human	24	3	2	[2.329–2.924] (2)	Human
Ae. albopictus	Human	36	0	5	[1.803–1.868] (2)	Human
					[1.086–1.689] (3)	/
Ae. albopictus	Human	48	0	5	[1.056–1.752] (5)	/
Ae. albopictus	Human	60	0	5	[1.101–1.225] (5)	/
An. gambiae S	Horse	1	3	2	[2.712–2.885] (2)	Horse
An. gambiae S	Horse	12	3	2	[2.683–2.859] (2)	Horse
An. gambiae S	Horse	24	3	2	[2.607–2.726] (2)	Horse
An. gambiae S	Horse	36	0	5	[2.172–2.481] (2)	Horse
					[1.185–1.302] (3)	/
An. gambiae S	Horse	48	0	5	[1.152–1.375] (5)	/
An. gambiae S	Horse	60	0	5	[1.141–1.403] (5)	/
An. gambiae S	Sheep	1	3	2	[2.532–2.541] (2)	Sheep
An. gambiae S	Sheep	12	3	2	[2.497–2.563] (2)	Sheep
An. gambiae S	Sheep	24	3	2	[2.464–2.478] (2)	Sheep
An. gambiae S	Sheep	36	0	5	[1.573–1.689] (5)	/
An. gambiae S	Sheep	48	0	5	[1.174–1.304] (5)	/
An. gambiae S	Sheep	60	0	5	[1.221–1.671] (5)	/
An. gambiae S	Mouse	1	3	2	[2.371–2.593] (2)	Mouse
An. gambiae S	Mouse	12	3	2	[2.455–2.476] (2)	Mouse
An. gambiae S	Mouse	24	3	2	[2.363–2.530] (2)	Mouse
An. gambiae S	Mouse	36	0	5	[0.984–1.234] (5)	/
An. gambiae S	Mouse	48	0	/	[–]
An. gambiae S	Mouse	60	0	/	[–]
An. gambiae S	Rat	1	3	2	[2.252–2.612] (2)	Rat
An. gambiae S	Rat	12	3	2	[2.285–2.360] (2)	Rat
An. gambiae S	Rat	24	3	2	[2.298–2.555] (2)	Rat
An. gambiae S	Rat	36	0	5	[1.339–1.681] (5)	/
An. gambiae S	Rat	48	0	5	[1.333–1.621] (5)	/
An. gambiae S	Rat	60	0	5	[1.154–1.343] (5)	/
An. gambiae S	Rabbit	1	3	2	[2.487–2.723] (2)	Rabbit
An. gambiae S	Rabbit	12	3	2	[2.524–2.733] (2)	Rabbit
An. gambiae S	Rabbit	24	3	2	[2.486–2.497] (2)	Rabbit
An. gambiae S	Rabbit	36	0	5	[1.189–1.633] (5)	/
An. gambiae S	Rabbit	48	0	5	[1.166–1.487] (5)	/
An. gambiae S	Rabbit	60	0	5	[1.245–1.504] (5)	/
An. gambiae S	Dog	1	3	2	[2.621–2.802] (2)	Dog
An. gambiae S	Dog	12	3	2	[2.590–2.651] (2)	Dog
An. gambiae S	Dog	24	3	2	[2.589–2.640] (2)	Dog
An. gambiae S	Dog	36	0	5	[2.590] (1)	/
An. gambiae S	Dog				[1.515–1.627] (4)	/
An. gambiae S	Dog	48	0	5	[1.279–1.604] (5)	/
An. gambiae S	Dog	60	0	5	[1.248–1.516] (5)	/
An. gambiae S	Non-engorged	/	3
Ae. albopictus	Non-engorged	/	3
Total			78	158

^aMosquitoes were collected from 1 to 60 h following blood meals and their abdomen protein extracts were submitted to MALDI-TOF MS
^bDatabase was composed of MS spectra from abdomen protein extract of An. gambiae S fed either on human, horse, sheep, mouse, rabbit or dog bloods, and from Ae. albopictus specimens fed on human and collected every 12 from 1 to 24 h
^cInto brackets are indicated the number of specimens included in each range of LSVs (upper and lower than 1.8), MS results with LSVs lower than 1.8 are italicized
^dVertebrate species blood origin are indicated only for specimens with LSVs upper than 1.8. LSVs, log score values

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ISSN: 1475-2875

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