Fig. 2
From: Biochemical and functional characterization of Plasmodium falciparum DNA polymerase δ
SDS–PAGE and western blot analysis of PfPolδS expression. a SDS–PAGE (10 %) and b western blot analysis of PfPolδS. Anti-His antibody was used for western blot analysis. Lane M molecular weight markers; lane 1 positive control vector with lacZ gene containing His6 sequence; lane 2 non-induced whole cell of E. coli BL21 carrying pET vector; lane 3 non-induced whole cell of E. coli BL21 carrying PfPolδS vector; lane 4 induced whole cell of E. coli BL21 carrying PfPolδS vector; lane 5 HisTrap HP column purified protein