Fig. 3From: Biochemical and functional characterization of Plasmodium falciparum DNA polymerase δAnalysis of recombinant PfPCNA1 expression by 12 % SDS–PAGE. Gel was stained with Coomassie blue R250. Lane M molecular weight markers; lane 1 non-induced whole cells of E. coli JM109 expression host; lane 2 total soluble protein from 1 mM IPTG-induced E. coli JM109 carrying PfPCNA1-constructed vector; lanes 3–7 elution fractions from Ni–NTA agarose purified proteinBack to article page