Table 3 Key outputs and features of the different analysis tools

Ease of use

 Free

✔

✔

✔

 96-well format^a

✔

✔

 Batch analysis of multiple drugs

✔

✔

✔

✔

 Batch analysis of multiple isolates

✔^e

✔

✔

✔

 Offline processing

✔

✔

✔

Key output

 IC₅₀ estimate

✔

✔

✔

✔

✔

✔

 Slope estimate

✔

✔

✔

✔

✔

 Confidence interval of estimates

✔

✔

✔

✔

✔

 Success criteria and warning signs

✔

✔

✔

✔

 Dose–response curve

✔

✔

✔

✔

✔

Data processing and analysis

 Data conversion

Critical

Critical

Easy

Critical

Easy

 Approximate time needed for data conversion (seconds)^{b, c}

45

25

25

25

25

 Data upload/entry

Critical

Critical

Easy

Critical

Easy

 Approximate time needed for data upload and processing (seconds)^{b, d}

120

15

20

300

90

 Approximate total time needed to obtain estimates (seconds)

165

40

45

325

115