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Table 3 Key outputs and features of the different analysis tools

From: Analysis of ex vivo drug response data of Plasmodium clinical isolates: the pros and cons of different computer programs and online platforms

  Method
  NONMEM WinNonlin GraphPad Prism 6.0 HN-Nonlin ICEstimator 1.2 IVART
Ease of use
 Free    
 96-well formata     
 Batch analysis of multiple drugs   
 Batch analysis of multiple isolates e   
 Offline processing    
Key output
 IC50 estimate
 Slope estimate  
 Confidence interval of estimates  
 Success criteria and warning signs   
 Dose–response curve  
Data processing and analysis
 Data conversion   Critical Critical Easy Critical Easy
 Approximate time needed for data conversion (seconds)b, c   45 25 25 25 25
 Data upload/entry   Critical Critical Easy Critical Easy
 Approximate time needed for data upload and processing (seconds)b, d   120 15 20 300 90
 Approximate total time needed to obtain estimates (seconds)   165 40 45 325 115
  1. aBased on the capability to enter the data in 96-well format
  2. bBased on the capability to run one isolate with five different drugs in duplicates distributed on two different 96-well plates
  3. cTime measurement includes the time needed to transfer raw data into a ‘converter’ spreadsheet and double checking the converted data to ensure that raw data was correctly entered and converted
  4. dTime measurement includes the time needed to transfer the converted raw data into the corresponding programs until the outputs were successfully transferred into a separate analysis spreadsheet
  5. eFor NONMEM, data from all isolates are modelled simultaneously