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Table 2 Specificity test of iiPCR method

From: Development of insulated isothermal PCR for rapid on-site malaria detection

Organism Fluorescence (550 nm) Detection
Before After Ratio
P. falciparum 32.111 81.552 2.540 +
P. knowlesi 30.803 85.508 2.776 +
P. vivax 30.396 80.878 2.661 +
P. malariae 30.413 43.614 1.434 +
P. ovale 31.760 86.672 2.729 +
P. coatneyi 30.640 51.410 1.678 +
P. cynomolgi 31.202 46.538 1.492 +
P. fieldi 30.574 52.506 1.717 +
P. inui 31.002 52.548 1.695 +
Human 32.108 31.869 0.993
A. caviae 31.688 32.761 1.034
A. aquariorum 31.288 31.125 0.995
A. hydrophila 31.648 32.532 0.983
V. parahaemolyticus 30.125 29.619 1.028
V. harveyi 30.344 29.875 1.020
S. marcescens 32.348 32.985 0.985
NTC 31.445 32.071 1.020
NTC 30.315 29.423 0.971
NTC 31.388 30.462 0.971
NTC 30.791 31.463 1.022
NTC 30.478 30.183 0.990
  1. ‘+’ positive detection, ‘−’ negative detection, NTC no template control