Table 2 Analyses of PCR products (COX-III single direct-PCR) of Plasmodium spp positive-controls and dilution performance of simulated mixed infections

Plasmodium spp. control (positive) samples

 P. falciparum (HB3 strain)

99.8

0.0

 P. vivax (AMRU1 strain)

100

0.0

 P. malariae (From the field)

99.8

0.0

 P. ovale wallikeri (I.D 5463)

99.3

0.0

 P. ovale curtisi (From the field)

98.4

3.2E−113

 P. knowlesi (Malayan Strain)

100

8.1E−180

Serial dilutions of mixed samples in ng (higher concentration of P. vivax Miami strain)

 3.3 E−01 P. vivax + 1.1E−06 of P. falciparum

98.5 P. vivax

2.9E−93

 5.5 E−02 P. vivax + 7.14E−06 of P. falciparum

 9.2 E−03 P. vivax + 4.2E−05 of P. falciparum

 1.5 E−04 P. vivax + 2.5 E−04 of P. falciparum

Serial dilutions of mixed samples in ng (higher concentration of P. falciparum HB3 strain)

 2.5 E−04 P. vivax +1.5 E−04 of P. falciparum

99.5 P. falciparum

9.4E−100

 4.2E−05 P. vivax + 9.2 E−03 of P. falciparum

 7.14E−06 P. vivax + 5.5 E−02 of P. falciparum

 1.1E−06 P. vivax + 3.3 E−01 of P. falciparum