Human malaria diagnosis using a single-step direct-PCR based on the Plasmodium cytochrome oxidase III gene

Malaria Journal

Table 3 Comparison of malaria diagnosis on 21 positive samples based on the COX-III single direct-PCR versus the 18s-rRNA nested PCRs

Sample code	PCR fragment of COX-III	PCR fragment of 18s-rRNA		Agreement
Sample code	PCR fragment of COX-III	Genus	Species	Agreement
IN01	P. malariae	+	Negative^a	No
IN02	P. falciparum	+	P. falciparum	Yes
IN03	P. falciparum	+	P. falciparum	Yes
IN04	P. falciparum	+	P. falciparum	Yes
IN05	P. vivax	+	P. vivax	Yes
IN06	P. vivax	+	P. vivax	Yes
IN07	P. vivax	+	Mixed infection^b	No
IN08	P. falciparum	+	P. falciparum	Yes
IN09	P. vivax	+	P. vivax	Yes
IN10	P. falciparum	+	P. falciparum	Yes
IN11	P. vivax	+	Mixed infection^{b, c}	No
IN12	P. vivax	+	Mixed infection^b	No
IN13	P. malariae	+	P. malariae	Yes
IN14	P. falciparum	+	Mixed infection^b	No
IN15	P. ovale curtisi	+	P. ovale	Yes
IN16	P. malariae	+	Negative^a	No
IN17	P. vivax	+	P. vivax	Yes
IN18	P. vivax	+	P. vivax	Yes
IN19	P. malariae	+	Negative^a	No
IN20	P. falciparum	+	P. falciparum	Yes
IN21	P. falciparum	+	P. falciparum	Yes

^a Negative results was obtain in two independent experiments
^b Mixed infections were due to P. falciparum and P. vivax
^c A second technical replicate of this sample was showed as a mono-infection with P. vivax

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