Fig. 1

Representative flow cytometry dot plot and gating hierarchy used to define B cell sub-types using cell markers CD3/14, CD19, CD10, CD38, CD27, and IgD. Using these markers, six CD19+B cell sub-sets are defined as: transitional (CD10⁺ CD27⁻ IgD⁺), naïve (CD10⁻ CD27⁻ IgD⁺), innate-like MBCs (CD10⁻ CD27⁺ IgD⁺), classical MBCs (CD10⁻ CD27⁺ IgD⁻), atypical MBCs (CD10⁻ CD27⁻ IgD⁻), and plasmablasts/plasma cells (CD10⁻ CD27^hi CD38^hi IgD⁻)