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Table 3 qPCR assay performance

From: New molecular settings to support in vivo anti-malarial assays

Gene

Slope

Y-Inter

R2

% Eff

P. falciparum

LDH

−3.77

27.03

0.99

84.33

CytB

−3.80

16.49

0.99

83.38

18S rRNA

−3.56

5.69

0.99

90.92

P. yoelii

LDH

−3.45

21.62

0.95

94.88

CytB

−3.70

13.97

1.00

86.28

18S rRNA

−3.73

16.65

0.99

85.49

P. berghe

LDH

−4.27

23.30

0.96

71.43

CytB

−5.64

22.53

0.92

50.46

18S rRNA

−4.55

25.42

1.00

65.93

  1. The efficiency of each qPCR was calculated with the formula: Efficiency = −1 + 10(−1/slope) (http://www.thermoscientificbio.com/webtools/qpcrefficiency/), which uses the slope produced by a qPCR standard curve. qPCR efficiency was validated by comparing the slope of the standard curve to the theoretical optimum of −3.32 which reflects 100 %