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Fig. 2 | Malaria Journal

Fig. 2

From: Endogenously-expressed NH2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands

Fig. 2

Sindbis virus production in BHK21 cells and mRNA expression in dsSindbis virus infected mosquito. a Light microscope observation of a BHK21 cell monolayer (top left panel), BHK21 stably transfected with Sin-PfRTSR (top right panel) or dsSin-EGFP (bottom left panel) and fluorescence microscopy of BHK-21 transfected with dsSin-EGFP (bottom right panel). b Detection of transcript from dsSindbis virus-infected mosquitoes. Total RNA samples were extracted from female mosquitoes eleven days after thoracic microinjection with each of the Sin-EGFP, Sin-PgpNT, Sin-PgRTSR, Sin-PgpTSR, Sin-PfpNT, Sin-PfRTSR viral particles, and used in a RT-PCR reaction. The same RNA samples were also analysed by actin-specific primers (Additional file 1). Abbreviations: C negative control (without template)

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