Fig. 1From: Generation of Plasmodium falciparum parasite-inhibitory antibodies by immunization with recombinantly-expressed CyRPAExpression of recombinant PfCyRPA. Glycosylated and non-glycosylated recombinant PfCyRPA (G-CyRPA and N-CyRPA, respectively) were expressed as His-tagged proteins in HEK cells and purified by affinity chromatography. Proteins were analysed by denaturing/reducing gel electrophoresis (SDS-PAGE) and stained with Coomassie blue (a), and identified by western blot analysis with the PfCyRPA-specific mAb c12 [32] (b)Back to article page