Fig. 2

Anti-PfCyRPA IgG responses in outbred NMRI mice. Anti-PfCyRPA IgG immune responses were determined 2 weeks after the third immunization. Mice were immunized either with glycosylated (G-) or non-glycosylated (N-) recombinant PfCyRPA. a Serum IgG endpoint titers were measured using ELISA plates coated with recombinant G-CyRPA. As control, serum IgG endpoint titers were also measured using ELISA plates coated with an unrelated hexa-His tagged protein (MUL_3720). Horizontal lines designate geometric means and each data point represents one animal. Data were processed and analysed using GraphPad Prism 6.0 (GraphPad). b Pre-immune (upper panel) and immune (lower panel) serum samples of individual mice were tested by Western-blotting analysis on P. falciparum 3D7 schizont-stage parasites under non-reducing conditions. The anti-PfCyRPA mAb c12 and the anti-GAPDH mAb 1.4a were used as positive control [32, 51]; the secondary antibody without primary antibody served as negative control