Fig. 4

Stage specific expression of PfCyRPA in late asexual blood stage parasites. a Indirect immunofluorescence staining of synchronized asexual blood stage parasites showed stage specific expression of PfCyRPA in schizont stages and free merozoites. Methanol/acetone fixed P. falciparum 3D7 parasites were probed with the anti-GAPDH mAb (green) and the anti-PfCyRPA mAb SB3.3 (red) as representative example. Nuclei were stained with DAPI (blue). Exposure times were identical for all pictures of the same channel. b Western blot analysis with lysates of tightly synchronized P. falciparum 3D7 blood-stage parasites with anti-PfCyRPA mAb SB1.6 (upper panel). The blot was probed for equal loading with an anti-GAPDH 1.4a mAb (lower panel). 4, 12, 20, 28, 36, 44 h post-invasion; M free merozoites