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Fig. 2 | Malaria Journal

Fig. 2

From: Identification of Plasmodium falciparum specific translation inhibitors from the MMV Malaria Box using a high throughput in vitro translation screen

Fig. 2

In vitro translation assay drug screens. a Lysates were incubated in the presence of anti-malarials and cycloheximide, a general eukaryotic translation inhibitor, all at 1 μM final concentration. Error bars represent the standard deviation among three biological replicates. b MMV Malaria Box compounds were added to lysates at a 1 μM final concentration. The average of three biological replicates were used to determine the extent of translation inhibition and normalized to the average of the DMSO controls present in each plate. Each point on the graph is the averaged response of a single drug. Each point is colour-coded by type of effect: top hit compound, no P. falciparum inhibition, high standard deviation, or inhibition in rabbit reticulocyte. The histogram on the right of the graph displays the total percentage of compounds with the given luciferase ratio

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