Fig. 3

Flow diagram and results of the Malaria Box screen. Starting with 400 compounds, each compound was tested in three independent biological replicates in in vitro translation assays with 1 μM of the compound. Of the 46 compounds that achieved at least 20 % inhibition of translation, only eight passed both the standard deviation filter, and specificity filter removing general eukaryotic translation inhibitors (as measured by translation inhibition in rabbit reticulocyte lysate) and luciferase inhibitors. a–h Structures of the eight compounds that passed the primary and secondary screens. The letter of each structure is matched to the compounds listed in Table 1