Fig. 1

PH1 and PH2 expression is intrinsically toxic to CH1305 yeast. a Colony formation assay in which cells were plated on non-inducing media (SD/-URA, top) or inducing media lacking CQ (SGR/-URA, bottom). Expression of PH1 and PH2 PfCRT causes slowed growth in yeast in the absence of drug that is substantially mitigated by targeting increased expression to the PM/decreased expression at the vacuole. b Intrinsic growth delay calculated by taking the difference in time needed to reach maximal growth rate in inducing vs non-inducing media. Results reported relative to control yeast expressing HB3 PfCRT, Baro et al. [17]