Table 1 List of primers used for plasmid construction
Amplified region | Forward primer | Reverse primer |
|---|---|---|
PyCSP promoter | CTATCAAATAATGTACTGCCCTTAAAAGC | GCTAATTTTCTCATCATTTTAAATATGTGTGTGTATATATAAG |
PfCSP ORF | CATATTTAAAATGATGAGAAAATTAGCTATTTTATCTG | aaagtcgacGTTGTTCTTAATGATTCAATGCACGGTG |
Three regions of PyCSP gene | aaaggatccGTATTGTGAACTTTCCTCATTTATTACG | aaagcggccgcCATATTTATGTACACCCTTTTGTGGACC |
PyCSP primer forward | CTACGTAACAAATATGCAAGATGG | Â |
PyCSP primer reverse | Â | CATATCCTGGAAGTAGAGAATCAAC |
PyCSP primer reverse | Â | AGAACCCTTGTGTTTGACGAAC |