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Table 2 Summary of treatments, number of independent infections, rationales, and classifications in the chemotaxis experiment

From: Ecological influences on the behaviour and fertility of malaria parasites

Treatment N Rationale Description
Uninfected RBC 10 Control. To test whether microgametes are attracted to a zone of cells, regardless of cell type Fresh blood
Live asexuals 13 Control. To test whether microgametes are attracted to parasites/parasitized RBC Purified asexual- stages in ookinete media
Lysed asexuals 12 Control. To test whether microgametes are attracted to material liberated from lysed parasites Asexual–stage parasites were cultured in ookinete media for 3 h before lysis (to match conditions used to prepare the lysed female treatment group)
Live females 9 Female. To test if microgametes are attracted to live female gametes Purified female gametes in ookinete media
Lysed females 10 Female. To test if microgametes are attracted to material liberated from lysed females Female gametes cultured in ookinete media for 3 h to allow for the production and secretion of chemotactic cues as well as all other cell contents, before being lysed
  1. The analysis involved comparing the effect of individual treatments as well as comparing treatments grouped into ‘control’ versus ‘female’