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Fig. 2 | Malaria Journal

Fig. 2

From: Characterization of an A-kinase anchoring protein-like suggests an alternative way of PKA anchoring in Plasmodium falciparum

Fig. 2

Pfakal expression levels in infected red blood cells. RT-qPCR analyses were performed on synchronized P. falciparum asexual blood stages. The pfakal transcript abundance was calculated using the 2−ΔΔCt method where Ct is the threshold cycle, and \(\Delta \Delta {\text{Ct}}\,{ = }\left( {{\text{Ct}}\,pfakal{\mathbf{ - }}{\text{Ct}}\,p90} \right)_{{{\text{stage}}\,{\text{x}}}} {\mathbf{ - }}\left( {{\text{Ct}}\,pfakal{\mathbf{ - }}{\text{Ct}}\,p90} \right)_{{{\text{late}}\,{\text{trophozoite}}}}\). Expression levels were normalized to pfakal gene transcription level in late trophozoites, the maximal expression observed throughout the erythrocyte cycle. The result for each sample was expressed by calculating the mean and standard deviations (bars on histograms) between the three experiments. A statistical analysis was performed with a Kruskal–Wallis test (p = 0.0077)

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