Fig. 5
Interaction between GST-PfPKA-R and (His)6-PfAKAL protein. a Nitrocellulose membrane coloration and anti-polyhistidine Western blot (b) after GST beads (2) and GST-PfPKA-R beads (8) were incubated in (His)6-PfAKAL solution. Molecular marker (1), empty lane (3), (His)6-PfAKAL (4), flowthrough after incubation of GST-PfPKA-R beads in (His)6-PfAKAL solution (5), beads washes (6–7). The recombinant GST-PfPKA-R and (His)6-PfAKAL proteins molecular weight are 75 and 32 kDa respectively. c A peptide array was performed using peptides derived from the PfAKAL whole protein sequence. An anti-polyhistidine western blot was realized to identify the peptides interacting with (His)6-PfPKA-R. d Gel filtration analysis of (His)6-PfPKA-R