Fig. 8
PfAKAL homology-based structural model and nucleotide binding studies, a Superposition of PfAKAL model with human AKAP18 central domain [PDB: 2vfl], represented with ribbon diagrams colored respectively in red and blue. b Superposition of PfAKAL model (red) with human AKAP18 that binds AMP [PDB: 2vfk] (blue). The residues involved in nucleotides binding are displayed as sticks, and AMP is colored in green. c Anti-polyhistidine western blot performed on precipitated protein complexes using cAMP- and AMP- agarose beads in (His)6-PfAKAL solution. (His)6-PfAKAL (1); beads washes (2–4), cAMP beads in (His)6-PfAKAL solution (5), AMP beads in (His)6-PfAKAL solution (6). d Anti-polyhistidine western blot analysis after incubation of GST-PfAKAL beads in (His)6-Pf14-3-3I solution in the presence (1) or absence (2) of 5 mM 3′–5′AMP; recombinants (His)6-PfAKAL and (His)6-Pf14-3-3I proteins molecular weight are 32 and 30 kDa respectively