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Fig. 2 | Malaria Journal

Fig. 2

From: Development of a Plasmodium berghei transgenic parasite expressing the full-length Plasmodium vivax circumsporozoite VK247 protein for testing vaccine efficacy in a murine model

Fig. 2

PvCSP(VK247) expression in PvCSP(VK247)/Pb sporozoites. a Replacement of the wild-type PbCSP gene with the PvCSP(VK210) gene or the PvCSP(VK247) gene in the PvCSP(VK210)/Pb or PvCSP(VK247)/Pb parasite lines was verified by PCR using genomic DNA from WT-Pb, PvCSP(VK210)/Pb, or PvCSP(VK247)/Pb, respectively. Pb 18s rRNA was used as a loading control. b The salivary glands from mosquitoes infected with the transgenic parasites were lysed and loaded onto 10 % gels, and then immunoblotted with the 3D11, 2F2, or 2E10E9 mAbs. c PvCSP(VK247)/Pb sporozoites were probed with the 2E10E9 mAb conjugated with Alexa Flour 488 (green). Parasite nuclei were visualized by DAPI (blue). Bar = 20 μm

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