Skip to main content
Fig. 5 | Malaria Journal

Fig. 5

From: Development of a Plasmodium berghei transgenic parasite expressing the full-length Plasmodium vivax circumsporozoite VK247 protein for testing vaccine efficacy in a murine model

Fig. 5

Sporozoite neutralization assay. PvCSP(VK247)/Pb sporozoites (2 × 103) were incubated with antibodies for 40 min on ice and then added to cultured HepG2 cells. After 72 h, the medium was removed and total RNA was isolated. Reverse transcriptase-PCR was performed to detect Plasmodium berghei 18s rRNA, which is liver-stage specific, and human β-actin was used as a control. Data represent two independent biological replicates

Back to article page