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Fig. 6 | Malaria Journal

Fig. 6

From: Cellular engineering of Artemisia annua and Artemisia dubia with the rol ABC genes for enhanced production of potent anti-malarial drug artemisinin

Fig. 6

Expression of key genes CYP71AV1 (Cytochrome P450), ADS (Amorpha-4, 11-diene, synthase), ALDH1 (Aldehyde dehydrogenase 1) involved in artemisinin synthesis pathway of Artemisia annua and Artemisia dubia for artemisinin biosynthesis, and TFAR1 (Trichome-Specific fatty acyl-CoA reductase 1) involved in trichome development and sesquiterpenoid biosynthesis. a. Expression of genes in leaves of Artemisia annua. A1, A2 and A3 represents three different transgenic lines of leaves, AC represents control shoots. b. Expression of genes in leaves of Artemisia dubia. D1, D2 and D3 represents three different transgenic lines of leaves, DC represents control shoots. c. Expression of genes in hairy roots, roots of transformed shoots of Artemisia annua. AH1 and AH2 represent two transgenic lines of hairy roots; AR1, AR2 and AR3 represent transformed roots of transgenic lines A1, A2 and A3 respectively. ARC represents control roots. d. Expression of genes in hairy roots, roots of transformed shoots of Artemisia dubia. DH1 and DH2 represent two transgenic lines of hairy roots; DR1, DR2 and DR3 represent transformed roots of transgenic lines D1, D2 and D3 respectively. DRC represents control roots. The actin gene was used as a house-keeping control. Both technical and biological replicates were performed. The average of three plants is shown together with error bars showing SD. Data analysed for significant difference at P < 0.001 with the control by Welch’s two samples t-test

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