Fig. 1
Experimental framework for testing anti-plasmodial activity of drug combinations. a Molecular structure of drugs used in the study. b Two types of infection protocols were used. For the in vivo/in vivo assay, mice were infected through the i.v. route with 107 Plasmodium parasitized red blood cells (pRBCs). One hour later, drugs were administered through the i.p. route, parasitaemia was monitored on thin blood smears over time, and mortality was recorded. c For the ex vivo/in vivo assay, Plasmodium parasitized red blood cells were exposed for 1 h at 37 °C to different drug combinations. They were then washed free of drugs, counted and naïve mice were infected with 105 treated pRBCs. Parasitaemia was monitored on thin blood smears over time, and mortality is recorded