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Fig. 2 | Malaria Journal

Fig. 2

From: Delayed acquisition of Plasmodium falciparum antigen-specific CD4+ T cell responses in HIV-exposed uninfected Malawian children receiving daily cotrimoxazole prophylaxis

Fig. 2

Representative FACS plots showing gating strategy for identifying antigen specific CD4+ T cell by intracellular cytokine staining assay. Heparinized whole blood collected at 6, 12 and 18 months was stimulated with PMA, P. falciparum 3D7 strain, purified protein derivative (PPD) and no antigen for 24 h. Lymphocytes were gated using FSC vs. SSC plots, FSC vs. CD3+ T cells and then CD4+T cells vs. CD8+T cells to isolate CD4+ T cells. Non-stimulated control samples were used to establish the threshold quadrants of background responses that were applied to quantify positive cytokine-producing cells in paired antigen-stimulated samples

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