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Table 1 Methodological summary of two-choice egg-count cage bioassays performed and references justifying the selection of test compounds

From: Gravid Anopheles gambiae sensu stricto avoid ovipositing in Bermuda grass hay infusion and it’s volatiles in two choice egg-count bioassays

Test substrate Conc of test substrate No of roundsa No of mosquitoes responding/laying eggs (total number tested) Literature references
EAD signalb Oviposition semio-chemical?c Detected in hay infusions
Hay infusion 0d 8e 75 (75)e    
10 % 3 126 (150)
25 % 3 22 (30)
50 % 3 25 (30)
100 % 3 28 (30)
3-methyl-1-butanol 0 5 209 (250) [44, 47, 67] [32]  
0.010 ppm 3 133 (151)
0.100 ppm 5 197 (250)
1.000 ppm 4 209 (300)
4-hepten-1-ol 0 9 185 (265)  
0.100 ppm 6 110 (148)
0.500 ppm 4 91 (119)
1.000 ppm 4 88 (116)
5.000 ppm 5 94 (149)
Phenol 0 9 185 (265) [43, 44, 67] [33] [25, 26]
0.100 ppm 5 107 (146)
0.500 ppm 3 110 (148)
1.000 ppm 3 71 (90)
5.000 ppm 5 102 (147)
Phenylmethanol 0 6 175 (210)   [32]  
0.500 ppm 5 144 (175)
1.000 ppm 5 138 (175)
2.500 ppm 5 149 (210)
5.000 ppm 6 169 (230)
4-methylphenol 0 11 319 (417) [4345, 67, 68] [33, 35] [25, 26]
0.100 ppm 5 130 (208)
0.500 ppm 4 87 (119)
1.000 ppm 5 171 (238)
5.000 ppm 5 109 (146)
2-phenylethanol 0 7 165 (200)   [32]  
0.100 ppm 4 162 (200)
0.500 ppm 5 125 (155)
1.000 ppm 5 131 (155)
2.500 ppm 4 144 (180)
5.000 ppm 6 168 (195)
Nonanal 0 4 360 (400) [44]   [26]
0.050 ppm 4 116 (150)
0.100 ppm 3 190 (250)
0.500 ppm 4 32 (60)
1.000 ppm 4 43 (60)
4- ethylphenol 0 6 169 (220) [44, 67] [33] [25, 26]
0.100 ppm 5 110 (148)
0.500 ppm 3 60 (80)
1.000 ppm 3 60 (87)
5.000 ppm 3 109 (132)
Indole 0 5 203 (430) [43, 44, 4649, 67, 69] [32, 43] [25, 26]
0.100 ppm 4 37 (60)
0.500 ppm 4 40 (60)
1.000 ppm 5 160 (225)
5.000 ppm 5 158 (205)
3-methylindole 0 8 195 (290) [43, 44, 67] [43] [25, 26]
0.010 ppm 8 102 (130)
0.100 ppm 8 98 (130)
0.500 ppm 8 110 (160)
1.000 ppm 8 170 (250)
  1. aA round equals a set of cages set up during the same experimental night with individual gravid female An. gambiae s.s. from one batch of mosquitoes and one batch of test substrates
  2. bReferences that report electro-antennographic detection (EAD) of An. gambiae s.s. to this specific compound
  3. cReferences that suggest (i.e., no bioassays performed) the specific compound to be an oviposition attractant/stimulant for Anopheles mosquitoes
  4. dZero (0) stands for untreated tap water, i.e., the test cups are filled with tap water (just as the control cups)
  5. eThe two equal-choice experiments used as reference for the hay infusion were not done in parallel to the hay infusion experiments (in contrast to all other experiments). For reference, data from 75 responders in equal-choice bioassays were randomly selected using an Excel add-in random sorter from a total of 375 responders from eight different rounds done prior to the infusion experiments