Table 1 PCR for anti-malarial resistance genes
From: Prevalence of anti-malarial resistance genes in Dakar, Senegal from 2013 to 2014
Gene | Primers | Amplicon (pb) | [MgCl2] (mM) | Tm (°C) | Elongation time (s) |
|---|---|---|---|---|---|
pfcrt | Forward CRT76-F | 182 | 4.5 | 55 | 20 |
TTG GTA AAT GTG CTC ATG TG T | |||||
Reverse CRT76-R | |||||
ACA AAT AAA GTT GTG AGT TTC GGA TG | |||||
pfmdr1-1 | Forward MDR1-1F | 610 | 2.5 | 52 | 30 |
AGA GAA AAA AGA TGG TAA CCT CAG | |||||
Reverse MDR1-1R | |||||
ACC ACA AAC ATA AAT TAA CGG | |||||
pfmdr1-2 | Forward MDR1-2F | 890 | 2.5 | 56 | 60 |
CAG GAA GCA TTT TAT AAT ATG CAT | |||||
Reverse MDR1-2R | |||||
CGT TTA ACA TCT TCC AAT GTT GCA | |||||
pfdhfr | Forward Dhfr-F | 562 | 2.5 | 52 | 20 |
ACG TTT TCG ATA TTT ATG C | |||||
Reverse Dhfr-R | |||||
TCA CAT TCA TAT GTA CTA TTT ATT C | |||||
pfdhps | Forward Dhps-F | 971 | 2.5 | 53 | 20 |
TTT TGT TGA ACC TAA ACG TG | |||||
Reverse Dhps-R | |||||
AAA CGT CAT GAA CTC TTA TTA GAT |