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Table 1 Polymorphisms, methods, restriction enzymes, primers, and band patterns used for investigation of SNPs in genes TNFA, IFNG, IL10

From: Cytokine gene polymorphisms are not associated with anti-PvDBP, anti-PvAMA-1 or anti-PvMSP-119 IgG antibody levels in a malaria-endemic area of the Brazilian Amazon

Polymorphisms in gene SNP Method enzimas annealing Primers Genotype Reference
IFN γ183
G > T
rs2069709 RFLP (53º)
Eco47I
FW: 5′-AAT GAT CAA TGT GCT TTG TG-3′
R: 5′-TAA GAT GAG ATG GTG ACAG-3′
TT: 271 pb
GT: 271 pb, 164 pb, 107pb
GG: 164pb, 107 pb
Suxia Qi et al. [20]
IFN γ +874
A > T
rs2430561 ASO-PCR (53º) CP: -5′-TCA ACA AAG CTG ATA CTC CA-3′
T : 5′-TTC TTA CAA CAC AAA ATC AAA TCT-3′
A: 5′-TTC TTA CAA CAC AAA ATC AAA TCA-3′
AA: 262 pb (reação A)
TT: 262 pb (reação T)
AT: 262 pb (reação A, T)
Medina et al. [21]
TNF α238
G > A
rs 361525 RFLP (60º)
MspI
FW: 5′-ATC TGG AGG AAG CGG TAG TG-3′
R: 5-AGA AGA CCC CCC TCG GAA CC’ 3′
AA: 156 pb
GG: 133 pb
GA: 153 pb, 133 pb
Hedayati et al. [22]
TNFα308
G > A
rs 1800629 RFLP (59º)
NcoI
FW: 5′-GAG GCA ATA GGT TTT GAG GGC CAT-3′
R: 5′-GGG ACA CAC AAG CAT CAAG 3′
AA: 147 pb
GG: 126 pb, 121 pb
GA: 147 pb, 126 pb, 21 pb
Asghar et al. [23]
TNFα1031
T > C
rs1799964 RFLP (57º)
BbsI
FW: 5′-TAT GTG ATG GAC TCA CCA GGT-3′
R: 5′-CCT CTA CAT GGC CCT GTC TT 3′
TT: 251 pb, 13 pb
TC: 251 pb, 180 pb, 71 pb e 13 pb
CC: 180 pb, 71 pb e 13 pb
Asghar et al. [23]
IL-10592
C > A
rs1800872 RFLP (54º)
RsaI
FW: 5′-GGG TGA GGA AAC CAA ATT CTC-3′
R:5′-GAG GGG GTG GGC TAA ATA TC 3′
AA: 240 pb, 77 pb, 36 pb e 08 pb
CC: 317 pb,36 pb e 08 pb
CA: 317 pb, 240 pb, 77 pb, 36 pb e 08 pb
 
IL-10819
C > T
rs1800871 RFLP (54º)
RseI
FW: 5′-GGG TGA GGA AAC CAA ATT CTC-3′
R: 5′-GAG GGG GTG GGC TAA ATA TC 3′
TT: 270 pb e 91 pb
CC: 217 pb, 91 pb e 53 pb
TC: 270 pb, 217 pb, 91 pb e 53 pb