Table 1 Polymorphisms, methods, restriction enzymes, primers, and band patterns used for investigation of SNPs in genes TNFA, IFNG, IL10
Polymorphisms in gene | SNP | Method enzimas annealing | Primers | Genotype | Reference |
|---|---|---|---|---|---|
IFN γ −183 G > T | rs2069709 | RFLP (53º) Eco47I | FW: 5′-AAT GAT CAA TGT GCT TTG TG-3′ R: 5′-TAA GAT GAG ATG GTG ACAG-3′ | TT: 271 pb GT: 271 pb, 164 pb, 107pb GG: 164pb, 107 pb | Suxia Qi et al. [20] |
IFN γ +874 A > T | rs2430561 | ASO-PCR (53º) | CP: -5′-TCA ACA AAG CTG ATA CTC CA-3′ T : 5′-TTC TTA CAA CAC AAA ATC AAA TCT-3′ A: 5′-TTC TTA CAA CAC AAA ATC AAA TCA-3′ | AA: 262 pb (reação A) TT: 262 pb (reação T) AT: 262 pb (reação A, T) | Medina et al. [21] |
TNF α −238 G > A | rs 361525 | RFLP (60º) MspI | FW: 5′-ATC TGG AGG AAG CGG TAG TG-3′ R: 5-AGA AGA CCC CCC TCG GAA CC’ 3′ | AA: 156 pb GG: 133 pb GA: 153 pb, 133 pb | Hedayati et al. [22] |
TNFα −308 G > A | rs 1800629 | RFLP (59º) NcoI | FW: 5′-GAG GCA ATA GGT TTT GAG GGC CAT-3′ R: 5′-GGG ACA CAC AAG CAT CAAG 3′ | AA: 147 pb GG: 126 pb, 121 pb GA: 147 pb, 126 pb, 21 pb | Asghar et al. [23] |
TNFα −1031 T > C | rs1799964 | RFLP (57º) BbsI | FW: 5′-TAT GTG ATG GAC TCA CCA GGT-3′ R: 5′-CCT CTA CAT GGC CCT GTC TT 3′ | TT: 251 pb, 13 pb TC: 251 pb, 180 pb, 71 pb e 13 pb CC: 180 pb, 71 pb e 13 pb | Asghar et al. [23] |
IL-10 −592 C > A | rs1800872 | RFLP (54º) RsaI | FW: 5′-GGG TGA GGA AAC CAA ATT CTC-3′ R:5′-GAG GGG GTG GGC TAA ATA TC 3′ | AA: 240 pb, 77 pb, 36 pb e 08 pb CC: 317 pb,36 pb e 08 pb CA: 317 pb, 240 pb, 77 pb, 36 pb e 08 pb |  |
IL-10 −819 C > T | rs1800871 | RFLP (54º) RseI | FW: 5′-GGG TGA GGA AAC CAA ATT CTC-3′ R: 5′-GAG GGG GTG GGC TAA ATA TC 3′ | TT: 270 pb e 91 pb CC: 217 pb, 91 pb e 53 pb TC: 270 pb, 217 pb, 91 pb e 53 pb |  |