An experimental hut evaluation of Olyset Plus, a long-lasting insecticidal net treated with a mixture of permethrin and piperonyl butoxide, against Anopheles fluviatilis in Odisha State, India

Table 3 Results of cone-bioassays

Sl no	Arms	Before any wash		After 20 washes		Prior to hut evaluation		After hut evaluation
Sl no	Arms	NE	CM (%)	NE	CM (%)	NE	CM (%)	NE	CM (%)
1	Untreated polyester net	50	0	50	0	50	2	50	4
2	Unwashed Olyset Net	50	100	–	–	50	100	50	100
3	Polyester net conventionally treated, washed until just before exhaustion	50	100	50	82^a (4 washes)	50	86	50	96
4	Olyset Net washed 20 times	50	100	50	62	50	100	50	100
5	Olyset Plus LLIN washed 20 times	50	100	50	90	50	100	50	100
6	Unwashed Olyset Plus LLIN	50	100	–	–	50	100	50	100

An. stephensi was used for cone-bioassays before any wash and after washes, whereas prior to hut evaluation and after hut evaluation, An. fluviatilis was used for the cone-bioassays
NE number of mosquitoes exposed, CM corrected mortality
^aWashed until just before exhaustion

ISSN: 1475-2875