Fig. 2From: Assessment of Pfs25 expressed from multiple soluble expression platforms for use as transmission-blocking vaccine candidatesSE-HPLC of Pichia and baculovirus Pfs25. a Pfs25 from Pichia (black line) and baculovirus (dashed red line) eluted predominantly as a monomer. Arrows indicate shoulder observed for Pichia Pfs25 that most likely represents a higher molecular weight component co-eluting with monomer. The shoulder present for Pichia Pfs25 could not be fully resolved and integrated. Molecular weight gel filtration standards (Bio-Rad, Hercules, CA) indicated a monomer, with Pfs25 eluting at its expected molecular weight (between 44 and 17 kDa). b Normalized absorbance for eluted monomer peaksBack to article page