Skip to main content

Table 2 Functional evaluation of antibodies against Baculovirus, Pichia and plant Pfs25 proteins

From: Assessment of Pfs25 expressed from multiple soluble expression platforms for use as transmission-blocking vaccine candidates

Sample Conca Mean Oocb % inhibitionc p valued
Experiment 1     
 Baculovirus 750 0.1 (0, 1) 100 (99, 100) <0.001
 Pichia 750 0 (0, 0) 100 (100, 100) <0.001
 Plant 750 0 (0, 0) 100 (100, 100) <0.001
 Plant (reduced) 750 25.1 (3, 45) 3 (−176, 67) 0.945
 Control 750 25.8 (0, 58) N/A N/A
Experiment 2     
 Baculovirus 250 0 (0, 0) 100 (100, 100) <0.001
  83 0 (0, 0) 100 (100, 100) <0.001
  28 0.4 (0, 2) 98 (94, 100) <0.001
 Pichia 250 0 (0, 0) 100 (100, 100) <0.001
  83 0 (0, 0) 100 (100, 100) <0.001
  28 0.3 (0, 2) 99 (95, 100) <0.001
 Plant 250 0 (0, 0) 100 (100, 100) <0.001
  83 0 (0, 0) 100 (100, 100) <0.001
  28 0.1 (0, 2) 100 (99, 100) <0.001
 Control 750 17.1 (0, 62) N/A N/A
  1. Groups of ten mice were injected with 10 µg of Pfs25 adjuvanted with montanide on days 0 and 21. On day 42 sera were pooled, and the purified IgGs were tested at the indicated concentrations by SMFA
  2. aIgG concentration (μg/ml) in a feeder
  3. bArithmetic mean (range) of oocysts intensity from 20 mosquitoes
  4. cPercent inhibition of mean oocyst intensity and the 95 % confidence interval (95 % CI)
  5. dTwo-sided p values for testing whether % inhibition is significantly different from 0