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Fig. 5 | Malaria Journal

Fig. 5

From: Behavioural and neurological symptoms accompanied by cellular neuroinflammation in IL-10-deficient mice infected with Plasmodium chabaudi

Fig. 5

CD4+ T cells exhibit intravascular localization in IL-10−/− animals infected with P. chabaudi. A 30 μm sagittal section of a female IL-10−/− (KO) brain 8 days post-inoculation with 105 Pcc-iRBCs i.p. Animals were injected with 2 × 106 CTV+ CD4 T cells i.p. 3.5 h before sacrifice, and 40 μg of DyLight488 labelled Lycopersicon esculentum (Tomato) Lectin i.v. 20 min before sacrifice without perfusion. Left panel Confocal image of an adoptively transferred CTV+ CD4+ T cell located in the Lectin+ microvasculature of the KO brain. Right panel Quantitation of adoptively transferred CTV+ CD4 T cells found inside or outside Lectin+ blood vessels within the KO brain. Imaging performed using a 20× objective with 4× digital zoom to visualize individual T cells within microvasculature

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