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Table 1 Summary of initial development experiments (experiments 1 and 2) for eave tubes, using the experimental house pictured in Fig. 1a

From: Eave tubes for malaria control in Africa: initial development and semi-field evaluations in Tanzania

Experiment

Question

Treatment(s)

Replicates

Measured

1a

How many mosquitoes will pass through open tubes over the course of a night?

1. Open tubes

3 nights total

Number of mosquitoes recaptured inside the house (out of 200 released)

1b

How effective are eave tubes placed at different heights?

1. 20 cm above the ground, treated with bendiocarb

2. 20 cm above the ground, untreated

3. 50 cm above the ground, treated with bendiocarb

4. 50 cm above the ground, untreated

5. 150 cm above the ground, treated with bendiocarb

6. 150 cm above the ground, untreated

7. 180 cm above the ground, treated with bendiocarb

8. 180 cm above the ground, untreated

3 nights per treatment

(24 nights total)

Total number of mosquitoes recaptured (out of 200 released)

1c

How effective are eave tubes with different diameters?

1. 10.16 cm in diameter tubes, treated with bendiocarb

2. 15.24 cm in diameter, treated with bendiocarb

3. 15.24 cm in diameter, untreated

3 nights per treatment

(9 nights total)

Total number of mosquitoes recaptured (out of 200 released)

1d

Does changing the angle of the tube impact the number of mosquitoes entering through the tubes?

1. Tubes placed at a horizontal angle

2. Tubes placed at an upward angle (highest end inside of the house)

3. Tubes placed at a downward angle (lowest end inside of the house)

Note: all treatments tested simultaneously using traps placed on the end of the tubes

9 nights total

Total number of mosquitoes captured inside the eave tube traps (out of 200 released)

1e

How effective are different bioactives in eave tubes?

1. Tubes screened with LLIN material

2. Tubes screened with untreated material

3. Tubes screened with bendiocarb-treated netting, wet formulation

4. Tubes screened with untreated material

5. Tubes screened with bendiocarb-treated netting, dry formulation

6. Tubes screened with untreated material

7. Tubes screened with entomopathogenic fungus (Beauveria bassiana).

8. Tubes screened with untreated material

Note: each comparison was pairwise between insecticide treatment and untreated material

Treatments 1–6:

3 nights per treatment

(18 nights total)

Treatments 7 and 8:

2 nights per treatment

(4 nights total)

Treatments 1–6: total number of mosquitoes recaptured (out of 200 released).

Treatments 7 and 8: proportion surviving (subsample of 50 recaptured mosquitoes)

2a

How does bendiocarb-treated material compare with LLIN material in eave tubes?

1. Eave tubes screened with bendiocarb-treated netting

2. Eave tubes screened with LLIN material

3. Eave tubes screened with untreated netting

7 nights per treatment (21 nights total)

Total number of mosquitoes recaptured (out of 200 released)

2b

How does bendiocarb-treated material in eave tubes compare to an LLIN used (with open eaves)?

1. Eave tubes screen with bendiocarb-treated netting

2. Open eaves, sleeper protected by an LLIN

3. Open eaves, sleeper protected by an untreated net

4 nights per treatment (12 nights total)

Total number of mosquitoes recaptured (out of 200 released)

  1. Eaves were closed and huts had no entry points for mosquitoes, unless otherwise noted. Each experiment used a human volunteer sleeping within the house for all treatments. Note that the word “effective”, as used in the wording of the question, depends on the measured outcome and respective controls