Characterization of fine specificity of the immune response to a Plasmodium falciparum rhoptry neck protein, PfAARP

Table 1 Phenotypic characterization of splenic CD4⁺ T cells and B cell in PfAARP and PBS immunized mice

Cell markers	Adjuvant control	Protein immunized	Fold change
B220⁺GL7⁺	0.34 ± 0.05	0.89 ± 0.49	2.50
B220⁺CD38⁺	24.5 ± 3.53	33.95 ± 7.70	1.38
B220⁺CD27⁺	0.6 ± 0.45	3.40 ± 2.18	5.67
B220⁺IgG1⁺	0.31 ± 0.13	0.55 ± 0.09	1.80
CD4⁺CD44⁺	22.35 ± 2.89	25.8 ± 0.84	1.15
CD4⁺CD11a⁺	9.68 ± 2.85	18.05 ± 1.9	1.86
CD4⁺CD62L⁺	28.45 ± 11.8	16.9 ± 0.56	0.59

Spleen cells from recombinant protein (test) and PBS (control) immunized mice were stained with respective labelled antibodies, acquired by FACS Calibur and analysed by FlowJo. Percent values with standard deviation and the fold change observed for the control and test mice are presented

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