Fig. 4

Phylogenetic relationships among the 20 Indian isolates sequenced and previously known Indian isolates of the Plasmodium vivax merozoite surface protein-3α (Pvmsp-3α) gene of the Indian isolates were compared to Belem strain (AF093584) was conducted using MEGA 6.0 using the reference strains and the maximum likelihood method, which is based on the Kimura two-parameter distance. Phylogenetic analysis of the Plasmodium vivax msp-3α gene. The phylogenetic tree for the 100 isolates, including 20 isolates from the present study of Pvmsp-3α, was constructed with maximum likelihood method based on the nucleotide sequences of the Indian isolates using the MEGA6 program. Numbers on the branches indicate bootstrap proportions (1000 replicates)