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Table 4 Immunoassays performed in the PRIMALVAC and PlacMalVac projects: exploratory objectives

From: Clinical development of placental malaria vaccines and immunoassays harmonization: a workshop report

PRIMALVAC

PlacMalVac

Exploratory objectives

Immunoassays

Exploratory objectives

Immunoassays

Antibody-induced inhibition of the interaction between iRBCPM expressing different VAR2CSA variants and CSA

BIA: Petri dishes and under flow conditions

Antibody-induced inhibition of the interaction between iRBCPM expressing different VAR2CSA variants and CSA

BIA: Petri dishes and 96-well automated method (static conditions)

Antibody cross-reaction with iRBCPM expressing different VAR2CSA variants

Flow cytometry

Antibody cross-reaction with iRBCPM expressing different VAR2CSA variants

Flow cytometry

Cytokines (large panel) produced after ex vivo stimulation of PBMCs with vaccine antigen

Flow cytometry (bead-based multiplex)

Cytokines (5 cytokines) produced after ex vivo stimulation of PBMCs with vaccine antigen

Flow cytometry (bead-based multiplex)

Antibody induced opsonic phagocytosis of iRBCPM expressing different VAR2CSA variants

Opsonic phagocytosis assay

  
  

Specific B lymphocyte memory

B cell ELISpot

  

IgG subclasses

ELISA