From: Clinical development of placental malaria vaccines and immunoassays harmonization: a workshop report
PRIMALVAC | PlacMalVac | ||
---|---|---|---|
Exploratory objectives | Immunoassays | Exploratory objectives | Immunoassays |
Antibody-induced inhibition of the interaction between iRBCPM expressing different VAR2CSA variants and CSA | BIA: Petri dishes and under flow conditions | Antibody-induced inhibition of the interaction between iRBCPM expressing different VAR2CSA variants and CSA | BIA: Petri dishes and 96-well automated method (static conditions) |
Antibody cross-reaction with iRBCPM expressing different VAR2CSA variants | Flow cytometry | Antibody cross-reaction with iRBCPM expressing different VAR2CSA variants | Flow cytometry |
Cytokines (large panel) produced after ex vivo stimulation of PBMCs with vaccine antigen | Flow cytometry (bead-based multiplex) | Cytokines (5 cytokines) produced after ex vivo stimulation of PBMCs with vaccine antigen | Flow cytometry (bead-based multiplex) |
Antibody induced opsonic phagocytosis of iRBCPM expressing different VAR2CSA variants | Opsonic phagocytosis assay | ||
Specific B lymphocyte memory | B cell ELISpot | ||
IgG subclasses | ELISA |