Fig. 3

Maximum likelihood tree of Thai Plasmodium falciparum populations based on msp-3 gene analysis. The tree was constructed by the MEGA program using msp-3 nucleotide sequences (nucleotide positions 232–783 after P. falciparum 3D7 strain). The sequences are identified with their names of parasite isolates. The letters indicate the origin of isolates: MH, Mae Hong Son; K, Kanchanaburi; RN, Ranong; TD, Trat; UB, Ubon Ratchatani. The tree was rooted using the sequence of the P. reichenowi msp-3 gene as an out group [41]. Bootstrap values of >50 % are shown. The scale bar indicates nucleotide substitutions per site