Fig. 2

Substrate preference of recombinant PfRuvB3. Substrate preparation and assay conditions were as described under “Preparation of DNA helicase substrates” and “DNA helicase activity assay”. Helicase assays were carried out by using 1 μg of purified PfRuvB3 at 37 °C 90 min. Each panel shows substrate used and autoradiogram on gel. DNA duplex substrates in panels a, b, c, d, e and f were short oligonucleotide, long oligonucleotide, blunted end, 5′ overhang, 3′ overhang and 3′, 5′ overhang, respectively. Pentagon denotes ³²P-labelled end. Percent unwinding is shown above each autoradiogram. Lanes C and H are control reaction without enzyme and heat-denatured substrate respectively. Lane E is reaction with recombinant PfRuvB3