Fig. 5From: Characterization of Plasmodium falciparum ATP-dependent DNA helicase RuvB3Time-dependent ATPase activity of recombinant PfRuvB3. ATPase activity of PfRuvB3 were assayed using single-stranded DNA (ssM13 mp18) under conditions as described in “PfRuvB3 ATPase activity assay”. ATPase assay was carried out at 37 °C under various incubation time ranging from 10 to 180 min. Control is ATPase assay without enzyme at 37 °C 180 min. a Autoradiogram. b Kinetics of ATPase activity of recombinant PfRuvB3. Result is shown as mean ± SD of three replicatesBack to article page