Fig. 7
From: Characterization of Plasmodium falciparum ATP-dependent DNA helicase RuvB3
Effects of helicase inhibitors on the ATPase activity of PfRuvB3. a Autoradiography of the thin layer chromatography plate. The negative control reactions were performed under standard condition without enzyme (Lane 1). Lane 2 is the assay reaction with enzyme, and lanes 3–5 are ATPase activity of PfRuvB3 in the presence of 50 μM of doxorubicin, daunorubicin and netropsin respectively. Lane 6 is the assay reaction with heat-inactivated enzyme. b quantification of the ATP hydrolysis in an absence and the presence of inhibitors