Serological markers to measure recent changes in malaria at population level in Cambodia

Kerkhof, Karen; Sluydts, Vincent; Willen, Laura; Kim, Saorin; Canier, Lydie; Heng, Somony; Tsuboi, Takafumi; Sochantha, Tho; Sovannaroth, Siv; Ménard, Didier; Coosemans, Marc; Durnez, Lies

doi:10.1186/s12936-016-1576-z

Malaria Journal

Table 1 Overview of the antigens (peptides and recombinant proteins) used in this study

From: Serological markers to measure recent changes in malaria at population level in Cambodia

Antigens	Sequence (N-terminal to C-terminal)	g/mol	Life-cycle stages	Plasmodium species	Peptide or recombinant protein	Ref.
CSP	NANPNANPNANPNANPNVDPNVDPC	2257.67	Sporozoite	P. falciparum	Peptide	[27]
Pf13	C-terminal His-tag produced in E. coli		Sporozoite	P. falciparum	Recombinant protein	[69, 70]
STARP.R	STDNNNTKTISTDNNNTKTIC	2299.42	Sporozoite and liver stage	P. falciparum	Peptide	[27, 71]
SALSA2	NGKDDVKEEKKTNEKKDDGKTDKVQEKVLEKSPKC	4019.52	Sporozoite and liver stage	P. falciparum	Peptide	[27, 71]
SR11.1	EEVVEELIEEVIPEELVLC	2213.50	Sporozoite and liver stage	P. falciparum	Peptide	[27, 71]
LSA1.41	LAKEKLQEQQSDLEQERLAKEKLQEQQSDLEQERLAKEKE KLQCERRAKEKLQEQQSDLEQRKADTKKC	5297.97	Liver stage	P. falciparum	Peptide	[27, 72, 73]
LSA1.J	ERRAKEKLQEQQSDLEQRKADTKKC	3046.43	Liver stage	P. falciparum	Peptide	[27, 71, 73]
LSA3.RE	VESVAPSVEESVAPSVEESVAENVEESVC	2991.20	Liver stage	P. falciparum	Peptide	[27, 71]
Pf.MSP1.19	Glutathione S-transferase (GST) fusion protein. C-terminal expressed in E. coli		Merozoite	P. falciparum	Recombinant protein	[38, 40]
GLURP	EDKNEKGQHEIVEVEEILC	2241.47	Trophozoite	P. falciparum	Peptide	[27, 71, 74]
Pf.GLURP.R2	C-terminal produced in E.coli		Trophozoite	P. falciparum	Recombinant protein	[27, 71, 75]
PvVK210.CSP	DGQPAGDRAAGQPAGDRADGQPAGDRADGQPAGC	3206.30	Sporozoite	P. vivax	Peptide	[71, 76, 77]
PvVK247.CSP	ANGAGNQPGANGAGNQPGANGAGNQPGANG AGNC	2905.95	Sporozoite	P. vivax	Peptide	[71, 76, 77]
PvCSP (chimera)	Soluble His-tag protein expressed in a wheat-germ cell free expression system		Sporozoite	P. vivax	Recombinant protein	[76, 77]
PvAMA1			Merozoite	P. vivax	Recombinant protein	[78, 79]
PvEBP			Merozoite	P. vivax	Recombinant protein	[80, 81]
PvDBP			Merozoite	P. vivax	Recombinant protein	[81]
Pv.MSP1.19	C-terminal produced in the baculovirus expression system		Merozoite	P. vivax	Recombinant protein	[38, 40, 48]
PmCSP	GNAAGNAAGNDAGNAAGNAAGNAAGNAAGNAAC	2358.37	Sporozoite	P. malariae	Peptide	[71]
SALIV1	EKVWVDRDNVYCGHLDCTRVATFC	2830.22	Salivary gland proteins	An gambiae	Peptide	[27, 82]
SALIV2	ATFKGERFCTLCDTRHFCECKETREPLC	3324.84	Salivary gland proteins	An gambiae	Peptide	[27, 82]

Ags are organized according to the Plasmodium species and the life-cycle stages in the human host

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ISSN: 1475-2875

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