Fig. 4

Inhibition of Pfnek-2 and Pfnek-2(V24C) kinase activity by NCL-00016066. a, b Representative experiment showing the effect of NCL-00016066 (10 μM) on the activity of Pfnek-2 and Pfnek-2(V24C). Shown is the autoradiograph (a) and Coomassie blue stained radioactive gel (b). c, d Same as (a, b) except the concentration of NCL-00016066 is 1 μM. The position of molecular weight markers (kDa) are shown. e, f Quantification of the experiments shown in (a–d). Data shown is the mean ± SEM; n = 3. Data analysed using Students paired t test *p ≤ 0.05. g Concentration–response curve for the activity of NCL-00016066 against Pfnek-2 and Pfnek-2(V24C). Data shown is the mean ± SEM; n = 3. Data analysed using students paired t-test *p ≤ 0.05