Fig. 5

Irreversible inhibition of Pfnek-2(V24C) activity by NCL-00016066. a, b Activity of NCL-00016066 (20 μM) against Pfnek-2 (a) and Pfnek-2(V24C) (b) before and after wash out of the inhibitor. Shown are the autoradiographs (a, b). The position of molecular weight markers (kDa) are shown. c Quantification of three independent experiments indicating the effect of washing on Pfnek-2(V24C). Data shown is the mean ± S.E.M; n = 3. Data analysed using Students paired t-test *p ≤ 0.05