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Fig. 6 | Malaria Journal

Fig. 6

From: A new tool for the chemical genetic investigation of the Plasmodium falciparum Pfnek-2 NIMA-related kinase

Fig. 6

Shift in molecular weight of Pfnek-2 V24C as determined by mass spectroscopy. Pfnek-2 wild-type without (black trace) and with (red trace) NCL-00016066 a show no differences in peaks of the mass to charge ratios (m/z) for any particular charge state and number of phosphate groups (Table 1). In contrast when the mutant is incubated with inhibitor (b), there is a shift to the right (red trace) compared to without inhibitor (black trace) indicative of an increase in mass of 315.03 ± 0.53 Da—the approximate weight of the inhibitor confirming covalent bonding of the inhibitor to cysteine 22. For the purposes of clarity, in each panel the red trace is shifted vertically with respect to the black trace

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