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Table 1 Molecular mass of Pfnek-2, Pfnek-2(V24C) and NCL-00016066

From: A new tool for the chemical genetic investigation of the Plasmodium falciparum Pfnek-2 NIMA-related kinase

M+ #phospho Charge state distribution MW (Da) Std Dev (Da) MW (calc) Δ (Da)
44+ 43+ 42+ 41+ 40+ 39+ 38+
Pfnek2+ vehicle
 5 882 903 924 947 971 995 1022 38,784 0.20 38,784 0.12
 6 884 905 926 949 973 998 1024 38,864 0.72 38,864 0.31
 7 886 907 928 951 975 1000 1026 38,944 0.68 38,944 0.30
 8 888 909 930 953 977 1002 1028 39,024 0.51 39,024 0.42
Pfnek2+ NCL-00016066
 5 882 903 924 947 971 995 1022 38,784 0.80 38,784 0.02
 6 884 905 926 949 973 998 1024 38,865 0.62 38,864 0.84
 7 886 907 928 951 975 1000 1026 38,944 0.75 38,944 0.43
 8 888 909 930 953 977 1002 1028 39,024 0.62 39,024 0.12
V24C+ vehicle
 5 883 903 924 947 971 995 1022 38,786 0.83 38,788 −1.58
 6 884 905 926 949 973 998 1024 38,866 0.58 38,868 −1.71
 7 886 907 928 951 975 1000 1026 38,946 1.27 38,948 −1.21
 8 888 909 930 953 977 1002 1028 39,028 2.12 39,028 0.23
V24C+ NCL-00016066
 5 890 910 932 955 979 1004 1030 39,103 1.77 38,788 315.30
 6 892 912 934 957 981 1006 1032 39,183 1.30 38,868 314.88
 7 893 914 936 959 983 1008 1034 39,262 1.35 38,948 314.35
 8 895 916 938 961 985 1010 1036 39,343 1.71 39,028 315.59
  1. Predicted molecular weight (Predicted MW) = mass of kinase + phosphorylation sites
  2. Δ (Da) = difference between predicted MW and measured MW for each charged state distribution
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Malaria Journal

ISSN: 1475-2875

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