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Table 1 Molecular mass of Pfnek-2, Pfnek-2(V24C) and NCL-00016066

From: A new tool for the chemical genetic investigation of the Plasmodium falciparum Pfnek-2 NIMA-related kinase

M+ #phospho

Charge state distribution

MW (Da)

Std Dev (Da)

MW (calc)

Δ (Da)

44+

43+

42+

41+

40+

39+

38+

Pfnek2+ vehicle

 5

882

903

924

947

971

995

1022

38,784

0.20

38,784

0.12

 6

884

905

926

949

973

998

1024

38,864

0.72

38,864

0.31

 7

886

907

928

951

975

1000

1026

38,944

0.68

38,944

0.30

 8

888

909

930

953

977

1002

1028

39,024

0.51

39,024

0.42

Pfnek2+ NCL-00016066

 5

882

903

924

947

971

995

1022

38,784

0.80

38,784

0.02

 6

884

905

926

949

973

998

1024

38,865

0.62

38,864

0.84

 7

886

907

928

951

975

1000

1026

38,944

0.75

38,944

0.43

 8

888

909

930

953

977

1002

1028

39,024

0.62

39,024

0.12

V24C+ vehicle

 5

883

903

924

947

971

995

1022

38,786

0.83

38,788

−1.58

 6

884

905

926

949

973

998

1024

38,866

0.58

38,868

−1.71

 7

886

907

928

951

975

1000

1026

38,946

1.27

38,948

−1.21

 8

888

909

930

953

977

1002

1028

39,028

2.12

39,028

0.23

V24C+ NCL-00016066

 5

890

910

932

955

979

1004

1030

39,103

1.77

38,788

315.30

 6

892

912

934

957

981

1006

1032

39,183

1.30

38,868

314.88

 7

893

914

936

959

983

1008

1034

39,262

1.35

38,948

314.35

 8

895

916

938

961

985

1010

1036

39,343

1.71

39,028

315.59

  1. Predicted molecular weight (Predicted MW) = mass of kinase + phosphorylation sites
  2. Δ (Da) = difference between predicted MW and measured MW for each charged state distribution