M+ #phospho
|
Charge state distribution
|
MW (Da)
|
Std Dev (Da)
|
MW (calc)
|
Δ (Da)
|
---|
44+
|
43+
|
42+
|
41+
|
40+
|
39+
|
38+
|
---|
Pfnek2+ vehicle
|
5
|
882
|
903
|
924
|
947
|
971
|
995
|
1022
|
38,784
|
0.20
|
38,784
|
0.12
|
6
|
884
|
905
|
926
|
949
|
973
|
998
|
1024
|
38,864
|
0.72
|
38,864
|
0.31
|
7
|
886
|
907
|
928
|
951
|
975
|
1000
|
1026
|
38,944
|
0.68
|
38,944
|
0.30
|
8
|
888
|
909
|
930
|
953
|
977
|
1002
|
1028
|
39,024
|
0.51
|
39,024
|
0.42
|
Pfnek2+ NCL-00016066
|
5
|
882
|
903
|
924
|
947
|
971
|
995
|
1022
|
38,784
|
0.80
|
38,784
|
0.02
|
6
|
884
|
905
|
926
|
949
|
973
|
998
|
1024
|
38,865
|
0.62
|
38,864
|
0.84
|
7
|
886
|
907
|
928
|
951
|
975
|
1000
|
1026
|
38,944
|
0.75
|
38,944
|
0.43
|
8
|
888
|
909
|
930
|
953
|
977
|
1002
|
1028
|
39,024
|
0.62
|
39,024
|
0.12
|
V24C+ vehicle
|
5
|
883
|
903
|
924
|
947
|
971
|
995
|
1022
|
38,786
|
0.83
|
38,788
|
−1.58
|
6
|
884
|
905
|
926
|
949
|
973
|
998
|
1024
|
38,866
|
0.58
|
38,868
|
−1.71
|
7
|
886
|
907
|
928
|
951
|
975
|
1000
|
1026
|
38,946
|
1.27
|
38,948
|
−1.21
|
8
|
888
|
909
|
930
|
953
|
977
|
1002
|
1028
|
39,028
|
2.12
|
39,028
|
0.23
|
V24C+ NCL-00016066
|
5
|
890
|
910
|
932
|
955
|
979
|
1004
|
1030
|
39,103
|
1.77
|
38,788
|
315.30
|
6
|
892
|
912
|
934
|
957
|
981
|
1006
|
1032
|
39,183
|
1.30
|
38,868
|
314.88
|
7
|
893
|
914
|
936
|
959
|
983
|
1008
|
1034
|
39,262
|
1.35
|
38,948
|
314.35
|
8
|
895
|
916
|
938
|
961
|
985
|
1010
|
1036
|
39,343
|
1.71
|
39,028
|
315.59
|
- Predicted molecular weight (Predicted MW) = mass of kinase + phosphorylation sites
- Δ (Da) = difference between predicted MW and measured MW for each charged state distribution